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Posts in category G. van Iterson jr

Material relating to van Iterson’s work and/or lab

The Delft School photograph collection

The Delft School of Microbiology has a rich collection of photographs. As I mentioned in an earlier post (23 September, 2020), we have recently been able to have many of them professionally scanned, thanks to a grant from the Prins Bernhard Cultuurfonds, and I thought that readers might like to see some of the results.

Before travel became so convenient, it was common to exchange photographs with colleagues and friends, especially if you were collaborating with them. As you can see from the photo below, Kluyver liked to exhibit some of these photos over his desk.

 

 

Some of those photos can be seen below.

 

Another common subject in the collection’s photographs features groups of people.

As many people who teach large numbers of students in a year will agree, the most efficient way to start each course is to photograph the group and add their individual names. Professor van Iterson photographed all of his practical courses, and attached each photograph to a transparency numbering the people, and a list of the names. 2 examples are shown below.

Photographs of small and large groups also occur frequently. The examples presented here show  small and larger conference photographs, Professor Kluyver and his staff, some time after World War II, Professor van Iterson on one of his visits to Indonesia, and Prins Bernhard being awarded an Honorary Doctorate by Professor Kluyver.

 

I must repeat my thanks to the Prins Bernhard Cultuurfonds for funding the scanning of our photo collection. Not only does it make research and publishing of the images easier, but we are currently packing up the collection to move to other buildings and the complete set of scanned images serves as a form of insurance against loss or damage during the moves.

 

And the research goes on!

A couple of readers have asked about whether research into different aspects of the Delft School will still be possible in the future, and the answer is yes, of course. We have had volunteers scanning the fragile papers and notebooks for years and, thanks also to a group of Delft students last summer, about half the papers have been scanned. Eventually, the rest of the papers will also be scanned and it will be possible to research everything from the scans without having to handle the (sometimes very fragile) original papers.

They will be in the best possible place – a purpose-built archive with professional archivists.

All good things…

The Beijerinck Museum and Delft School of Microbiology Archive has closed and will not reopen in its current form.
Delft University has sold the building that houses our current hosts, the Delft Science Centre, and the new Science Centre building does not have suitable space for us.
Much of the Archive will be going to Erfgoed Delft, previously the Delft City Archives. It’s the best way to ensure the safety of the papers and keep them in Delft, where they belong. The rest of the collection will be dispersed, that’s still under discussion.
Many thanks to everyone who has volunteered for different projects over the years, and to our many supporters.

Using the Zeiss-Abbe polarizing attachment for their 19th century microscopes

It’s now over a year since I described how to use the Abbe polarizing attachments with one of our late 19th century jug-handled microscopes. Since then, I’ve had one disappointment. The attachment does not fit any of our more “modern” (ie 20th century, with electricity) microscopes and I’ve had to continue using the mirrored model.

My original attempts to use them all employed thin mineral slices because I wanted samples with known, reliable reactions to the polarizing light, but then I started considering what they were actually used for in Microbiology or Botany laboratories.  We have at least 20 of the adaptors, plus a number of partial or damaged kits, so they must have originally been used for teaching before WW1. It seemed a good idea to test their performance with biological samples.

In all of the image pairs presented here, the first image is without polarization, and the second with it.

The first image set (above) shows two of my standard microscope-testing samples (very useful for comparisons). The insect is a lacewing and the sample came from one of its wings. Despite the fact that the lacewing has been dead at least 2 years (it was “rescued” from a spider’s web), the veins gave clear, silvery-white fluorescence with polarising light. The thin section is a commercially bought, stained section of a plant stem. From comparisons with other samples, it is the stains that fluoresce, not the fixed plant tissue.

The remaining images (above) were made with living algae (Closterium sp.) at 2 magnifications (Zeiss lenses C and D, but their magnifications need to be recalibrated because of the distance of the camera’s sensor from the lens). It appears that only sections of their chloroplasts were fluorescing. The sample had been taken a week previously from a pond in the area where the Berkelsemeer (Van Leeuwenhoek’s first microbial hunting ground) used to be.

Guessing from the location of the cupboards where we found the attachments, it seems likely that they belonged to the Botany department. We’ve recently found a few of their practical handbooks in the archive – maybe one day I’ll be able to try the attachments with the original experiments! First, I want to try the other microscopes in our collection.

 

Delft’s Science Centre nominated for Museum Prize!

Delft Science Centre with new Kamer van Beijerinck, 2nd floor with balcony

We’re very excited to be able to tell you that the Delft Science Centre, home of the Beijerinck, van Iterson and Kluyver collection as well as the Faculty of Mining’s mineral collection and more modern exhibits from Delft University of Technology, has been nominated for the BankGiro Lottery’s Museum Prize.

It would be very kind and much appreciated if you would support us by voting.

The voting site is in Dutch, so here’s a translation into English for those who use other languages.

1; Go to the site here:

https://www.museumprijs.nl/genomineerden/science-centre-delft

2; Hit the green button labelled “Stem op dit museum”.

3: When the blue rectangle comes up, it says “Yes, I’m voting for the Science Centre Delft” and asks you to fill in your name and then your email address (that’s so that they can make sure that each email address is linked to one vote and so that they can let you know if you win the prize trip to New York).

Also click the box immediately under the email box – it indicates that you’re over 18 and agree with the rules (which you can see by hitting the “actievoorwaarden” link. If you want to read them, copy the text and paste it into Google Translate or another translation app). The two boxes below, if clicked, let them send you news about the BankGiro Lottery (who’re sponsoring the prize) and the Prins Bernhard Culture Fund (also sponsors).

4: Click on the green button.

You will then be thanked for voting and they will send an email to the address you gave them. The email will ask you to click on another green button to confirm your vote (it prevents folk from getting carried away and making up email addresses to let them vote often).

Exploring the Delft School’s microscope collection – 1.

As I have mentioned before, the Delft School of Microbiology Archive has a small collection of old and unusual microscopes. We also have a range of attachments with varying (and sometimes unidentified) functions as well as two boxes of mysterious parts. One of my projects for this winter is to try as many of these things out as possible, and write a methods book for the collection.

I have started with two red boxes from Ernst Abbe’s time at Carl Zeiss Jena (CZJ). Abbe was one of the founding fathers of CZJ and the inventor of many microscope techniques that we take for granted today, including standardizing  lens quality. One of the boxes contains the parts necessary to convert a simple microscope for work with polarization, the other contains a camera lucida. Both were obviously routinely used in the times of Beijerinck, Van Iterson and Kluyver and we have enough of each for every student in a practical class to use them. None of the boxes included instructions, and nobody I’ve asked had tried to use either one. Since they turn up sometimes on auction sites, it seems to be worth outlining my findings here. I used my late 19th century jug-handled CZJ microscope.

 

The polarizer.

The box contains a polarizer (top right in the photo), a calibrated ring (centre) and an eyepiece (bottom left). It took me a while to realize that the polarizer is actually two pieces which unscrew. The piece with the lens fits into the filter ring under the condenser, and the ring screws on underneath the filter ring to stabilize the polarizer. The calibrated ring fits around, and the eyepiece over the ocular.

The contents of the polarizer box.

Using my usual LED lamp, I adjusted the mirror and condenser to give optimum light and then inserted a slide. Rather than microorganisms, for these experiments it was simpler to use samples guaranteed to give the dramatic colour changes associated with polarizing microscopy and so I chose mineral samples prepared for the microscope by Dr F Krantz of Bonn around the beginning of the 20th century (and also in our collection). The pairs of photos show the extremes of uncrossed and crossed polarized light paths for 3 different minerals.

 

The camera lucida

The box contains a ring which fits over the microscope’s ocular. Attached to the ring is an arm with a mirror at its end and a shorter, moveable arm supporting the prism that combines the images from the microscope and the mirror. The arm allows the prism to swing over or away from the ocular, allowing the microscope to be used with and without it (very useful for focusing and sample placing).

Initially, I found this attachment very frustrating because everyone I had discussed it with confidently said that it projected the sample’s image onto paper beside the microscope. This did not happen. It was only when I looked through the ocular to check the microscope’s focus and saw a ghostly pen superimposed on the sample that I realised that the projection was the other way round! Thus far, I am not very satisfied with my photographs of the combined images, but I’ll post a picture here when I’m happy with them. The problem is not in using the equipment, but in convincing the camera that it can focus on the pen and sample at the same time – I see that CZJ’s catalogues of the time also offer a drawing platform for use with their camera lucida which was presumably exactly the correct height. If anyone wants to try, I’ve had better results with cream coloured paper rather than bright white paper which tends to reflect more in the field of view. Reducing the light in the room also helps.

Van Iterson’s wall chart collection.

 

 

In the days before the easy projection of images, wall charts were the usual manner of illustrating lectures and practical classes. This photo shows the autumn 1941 practical on microscopical research of living plants and plant products, with wall charts in use on the back wall. Prof van Iterson can be seen standing in the far left corner of the room.

 

The Archive has a large collection of these wall charts, and I’ve previously shown some of the printed ones as well as some by Henriette Beijerinck. The original wall charts for Van Iterson’s Department were produced by at least 15 people, not all of whom signed their work with their full names. They range from beautiful watercolours of useful plants to diagrams and tables – everything a Professor of Applied Botany could need for his lectures.

 

Like Beijerinck and Kluyver, in those days before the discovery of DNA, Van Iterson was interested in heredity and variation, and we have a series of watercolours illustrating Mendelian genetics, gender dimorphism and mimicry.

 

 

Researching and cataloguing the wall chart collection was a great labour of love by Truus ten Hoopen-van Hulsentop. If anyone can tell us anything more about the various artists, we’d be delighted to hear from you.

Beijerinck’s office (and the neighbours)…

At long last, the Beijerinck Museum is ready for visitors – indeed several groups, including the Dutch Microscopy Society and guests from the Queckett Microscopical Club, have had sneak previews. Also known as “Beijerinck’s Office” (Kamer van Beijerinck), it will only be open to escorted groups as we don’t want to spoil the atmosphere by putting everything into locked display cabinets. Some of our microscopes are now on display in the foyer of the Mekelzaal, the main conference room of the Science Centre.

At the end of BioDay, a meeting organised to celebrate the  TUDelft’s 175th birthday, the Museum was re-opened by Professor Karel Luyben, Rector Magnificus of the University, ably (?) assisted by someone claiming to be Prof Beijerinck’s assistant…

This seems like a good moment to take a look at our new surroundings. We are housed on the second floor of the Delft Science Centre. The building was originally the Faculty of Mining. It’s very easy to feel at home here as, like Prof van Iterson’s section of our previous building, our new home dates from the early 20th century and the design is very similar. The view from our windows is a great improvement!

The main part of the Science Centre  displays the modern achievements of Delft University of Technology and a range of prototypes (including Delft’s famous solar powered car) can be seen as well as displays that can be operated by the visitors. You can try improving the shape of an aircraft wing or use the driving simulators, among many other things – the exhibition often changes. The robotics lab is ever popular, as are the workshops where school groups (among others) come to try things out. For example, the Dutch Microscopy Society will be running a public workshop (about how to make botanic preparations (Wollemia nobilis)  and look at them under the microscope) from 14:00-16:30 on 7th May (2017). Participants will only pay the Science Centre entrance price. The details are here:  www.sciencecentre.tudelft.nl/nl/bezoek/agenda/event/detail/gratis-microscopie-workshop/. It’s in Dutch but the site will allow you to copy the text for pasting into Google translate (which is always amusing….).

The TUDelft has always had a  number of internationally important collections which started life as the working tools and products of its research departments. Among them, the minerals collection from the Faculty of Mining (which dates from the middle of the 19th century) is remarkably beautiful. It is housed, in its original display cases, in rooms next door to the Beijerinck Museum and in cabinets in other public parts of the building. Like the Beijerinck Museum, the Minerals Collection is open to escorted groups.

Just around the corner from the Science Centre is Delft’s Botanic Garden. Founded by Prof van Iterson with support from Prof Beijerinck, it was set up to provide plants for research in the Department of Applied Botany and is 100 years old this year (2017). Apart from their permanent collection (which includes an apiary), they frequently have exhibitions ranging from pottery through products made from plant material to photography. Their website is here: http://www.botanischetuin.tudelft.nl/en/

The Science Centre’s website with opening times and other information is here http://www.sciencecentre.tudelft.nl/en/.

Delft, the Home of Microbiology

An exhibition at Van Leeuwenhoek’s resting place.

2017 is the 175th anniversary of the founding of the Polytechnic School that eventually became Delft University of Technology. The University is celebrating with a 175 day Lustrum, much of which will focus on the Life Sciences. It is also 100 years since Van Iterson founded the Delft Botanic Garden on the land behind his laboratory.

invite lustrum bit e

We are beginning the celebrations with a look at the history of microbiology and the biosciences in Delft, from the 17th to the 21st centuries. The exhibition will run until 26 February, 2017.

invite poster s

This will take the form of an exhibition in the area around Antoni van Leeuwenhoek’s grave in the Oude Kirk.

The text on the poster boards is in Dutch, but there are also English language handouts. It includes 20th century teaching microscopes, a cross section of an electron microscope and  3-D scanned replicates of a Van Leeuwenhoek microscope and the Delft telescope.

Facsimile Van Leeuwenhoek microscope and its 3-D scanned replicate

Facsimile Van Leeuwenhoek microscope and its 3-D scanned replicate

The exhibition offers a taster of the achievements of Delft microbiologists and introduces some of the people who helped and supported them. For example, we might never have heard of Antoni van Leeuwenhoek and his little animals without Reinier de Graaf, the doctor who introduced van Leeuwenhoek to the Royal Society of London, publishers of much of his work. Jacques van Marken not only brought Beijerinck to Delft to establish his industrial microbiology laboratory, he was also one of the people instrumental in creating the Department of Microbiology and Professor’s Chair for Beijerinck at what was then the Delft Polytechnic.

Many others have provided help, support and encouragement, but the silent contributor to the history of microbiology is the City of Delft itself. Many very well-studied microorganisms were found for the first time in samples from Delft’s canals, soil and from industrial sources, even now.


Over 300 years ago, before Van Leeuwenhoek found his little animals, the city was already a hotspot in scientific research-for example Stevin and de Groot dropped different lead balls off the tower of the New Church and proved that they hit the ground at the same time 3 years before Galileo did the same experiment from the tower of Pisa!

CIMG0145

Delft’s New Church

From “out of date junk” to “exciting and rare” – our microscope collection

At the moment, sorting out the cupboards before our move has become very exciting as I’ve reached the microscope collection. Much of it was stored in the 1950s when the Laboratory of Microbiology moved from its original building, and has rarely been disturbed since then. Some of the microscopes date from the late 19th century, and even some of the 20th century ones are more interesting than might be expected.

The youngest of the companies represented in our microscope collection is probably the least well-known, especially outside the Netherlands.

Bleeker Nedoptifa

Founded by Dr Caroline E. (Lili) Bleeker and Gerard Willemse in 1939, Nedoptifa rapidly became known for the high standard of their optical products. They began with the production of binoculars for the Dutch army, but production was interrupted by WW2. Most of their microscope production seems to have been after 1945, when the “Nedoptifa” name came into use. The company cooperated with the Nobel prize-winner, Frits Zernike, in the development of phase contrast microscopy and held his patent on phase contrast microscopes. Bleeker retired at the end of 1963, the company was eventually taken over by a Delft firm and then in 1978 the factory in Zeist was closed.

Kluyver’s group seems to have used the basic Nedoptifa microscope for teaching – we have quite a few of them. Most of them have the standard circular stage, but a few are square. We also have one of their very early binocular microscopes as well as monocular and binocular phase contrast microscopes. Most of them, with the exception of the binocular phase contrast microscope, seem to have been use in in the laboratory before 1955.

Note added later: Disappointingly, after a visit in mid-June from Peter Paul de Bruyn, an expert on Bleeker microscopes, it seems that the microscopes whose boxes proclaimed them to be phase contrast microscopes do not have the necessary lenses or fittings. They may appear as we complete the packing of the collection, but it’s beginning to look unlikely.

Carl Zeiss Jena

This famous old microscope manufacturer needs no introduction. In what is probably our most famous portrait of Prof Beijerinck, he is clearly using one of their “jug handled” microscopes. Others in our collection are in the Zeiss catalogues of the late 19th century.

We also have some more unusual examples of their work including a “horizontal microscope” (intended for examining living plants) and a binocular microscope fitted with a prism on the right ocular to aid the drawing of samples.

I really shouldn’t have favourites, but I must admit a fondness for the very heavy preparation microscope that turned up in a battered old wooden box on top of a cupboard. When it is taken out of the box, the eyepieces on the right flip up, giving a forerunner of our modern stereomicroscopes which can be found in the 1902 Carl Zeiss Catalogue. There are 2 sets of lenses and oculars. During dissections, the microscope can be moved backwards and forwards along the metal bar.

Early Zeiss preparation or dissecting microscope

Early Zeiss preparation or dissecting microscope

Ernst Leitz Wetzlar

Another famous old company, Leitz seems to have been a favourite with Professor van Iterson. Among the microscopes from this company are a “measuring microscope”, a preparation microscope with wrist supports and the microscope (with an extensive collection of extra attachments) that he used after he retired.

Other companies

Other microscope makers are mostly represented by single instruments, so I will only show two of the most spectacular. First is the interference microscope made by Cooke, Troughton & Simms from the Van Iterson collection. This microscope is accompanied by a set of extra attachments and filters.

And lastly, one of the most unusual microscopes in our collection, a reversed microscope made by Nachet & Fils of Paris. The sample lies on the stage and can be illuminated from above, with the observer’s light path underneath the stage. It appears in the 1898 Nachet catalogue. This microscope was given to the collection by Dr and Mrs ten Hoopen, both of whom worked in the Department of Applied Botany before volunteering to help with the Archive and Museum after they retired.

The Delft School of Microbiology Archive owes a huge debt to both of them. Truus undertook two major tasks. First she restored our enormous collection of glass negatives (see the blogpost “Glass negatives galore!”) after they all got damp during asbestos removal in the attic where they were stored. She then went on to research and catalogue our collections of original and printed botanic and microbiological wall charts (see blogposts “The art of Henriette Beijerinck” and “Educational Wall Charts – where are they now?”). Meanwhile, Hens catalogued the microscopes and other equipment (we have the most amazing range of pH meters, for example) in our collection.

Without Truus and Hens ten Hoopen, we would know a lot less about the Collection than we do.

Reversed light path microscope by Nachet et fils

Reversed light path microscope by Nachet et fils

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